Welcome to Dr. Kate Brilakis' Learning Portal
let's review
DNA...
step 1:
collecting DNA sample
step 6:
analyzing DNA fragments
Analyzing DNA using a DNA "Fingerprint"
one bp
=
base pair
we're using bacteriophage lambda DNA
step 3:
cutting DNA sample into fragments
using restriction endonuclease enzymes
step 4:
separation of DNA fragments using gel electrophoresis
step 2:
isolating DNA sample
we will use three REs:
PstI
EcoRI
HindIII
DNA Analysis
One Nucleotide
different restriction enzyme recognize and cleaves different palindromic restriction sites
step 5:
staining of DNA fragments
separated using gel electrophoresis