we're using bacteriophage lambda DNA
step 2:
isolating DNA sample
we will use three REs:
PstI
EcoRI
HindIII
DNA Analysis
let's review
DNA...
step 1:
collecting DNA sample
One Nucleotide
step 3:
cutting DNA sample into fragments
using restriction endonuclease enzymes
different restriction enzyme recognize and cleaves different palindromic restriction sites
step 6:
analyzing DNA fragments
step 4:
separation of DNA fragments using gel electrophoresis
step 5:
staining of DNA fragments
separated using gel electrophoresis
Analyzing DNA using a DNA "Fingerprint"
one bp
=
base pair
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